麻风患者血清Th1/Th2/Th17相关细胞因子检测

摘要

Objective: To detect the levels of Th1, Th2 and Th17 related cytokines in the patients withleprosy and to explore the role of those cytokines in leprosy. Methods: The serum levels of IL-1, IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ and IL-17A were detected by flow cytometric bead array in 12 newly reportedleprosy patients, 29 cured leprosy patients and 37 healthy controls. The differences between groups were comparedby Kruskal-Wallis test and Nemenyi test. Results: The levels of IL-1 and IL-2 in newly reported leprosypatients were higher than that in cured leprosy patients, with significant difference (P<0.005). The levelsof IL-6 in newly reported leprosy patients and cured leprosy people were higher than those in healthy controls(P<0.005). The levels of IFN-γ and IL-17A in newly reported leprosy patients were higher than thosein healthy controls (P<0.005). There was no difference in the levels of IL-4,IL-10 and TNF among newlyreported leprosy patients, cured leprosy patients and healthy controls. Conclusion: Th1, Th2 and Th17 relatedcytokines may be associated with the development of leprosy.%目的：检测麻风患者血清中Th1/ Th2/ Th17相关细胞因子水平,探讨其在麻风发病中的作用。方法：利用微量样本多重蛋白定量技术检测12例新发麻风患者,29例治愈麻风患者及37例正常人血清中IL－1、IL－2、IL－4、IL－6、IL－10、TNF、IFN－γ和IL－17A 水平。利用Kruskal－Wallis 和Nemenyi方法进行组间比较。结果：新发组患者血清IL－1和IL－2水平显著高于治愈组(P<0.005)；新发组及治愈组患者血清IL－6水平均显著高于对照组(P<0.005)；新发组患者血清IFN－γ和IL－17A 水平显著高于对照组(P<0.005)。新发组、治愈组和对照组IL－4、IL－10和TNF 浓度差异无统计学意义。结论： Th1/ Th2/ Th17某些相关细胞因子可能与麻风的发病有关。