白芍总苷对 Nc/Nga 小鼠特应性皮炎的保护作用

摘要

目的：：确定白芍总苷(Total Glucosides of Paeony,TGP)对小鼠特应性皮炎的保护作用。方法：利用2,4－二硝基氯苯( DNCB)建立Nc/Nga小鼠实验性特应性皮炎模型。30只小鼠分为3组：正常对照组,模型组,实验组( TGP治疗),每组10只。实验组连续灌胃用药2周,分别于第8 d、15 d测量各组小鼠背部皮损厚度和皮肤质量,并利用ELISA法分别检测第8 d、15 d血清中细胞因子IFN－γ, IL－4,IL－5和IgE的表达。结果：白芍总苷治疗第8 d、15 d,皮损厚度变薄及皮损皮肤质量明显减小(P<0.05),血清细胞因子IL－4、IL－5和IgE水平明显低于模型组,IFN－γ水平明显高于模型组(P<0.05)。结论：白芍总苷可能通过促进IFN－γ分泌,下调炎症因子IL－4、IL－5水平以及抑制IgE表达从而对小鼠特应性皮炎产生保护作用。%Objective:To determine the effect of total glucosides of paeony (TGP) on atopic dermatitis in mice. Methods:An atopic dermatitis model was established on the back of NC/Nga mice with DNCB. Thirty mice were divided into 3 groups:control group, model group and TGP group ( 10 mice in each group) . The mice of TGP group were treated by TGP for 14 days. The levels of serum cytokines including IL-4 and IL-5, IFN-γ and IgE were detected with ELISA kit at day 8th and 15th. Results:TGP could significantly decrease the skin thickness and weightness at 8 days and 15 days (P<0.05). The levels of IL-4,IL-5 and IgE were significantly decreased , while the level of IFN-γ was increased in TGP group, compared with other groups. Conclusion:TGP has protective effects on atopic dermatitis in mice, which may be through inhibiting produc￣tion of IgE, IL-4 and IL-5, and promoting the secretion of IFN-γ.