荧光定量PCR与ELISA法检测儿童肺炎支原体感染的方法比较

摘要

Objective To investigate the value of real-time FQ-PCR and ELISA in the diagnosis of mycoplasma pneumoni-ae(MP) infection in children. Methods FQ-PCR was used to detect the MP-DNA and ELISA was used to detect the MP-IgM antibody in a total of 1590 specimens of conventional throat swab or sputum and 2mL EDTA anticoagulation from the infants and children suspected with MP infection and underwent pediatric outpatient and hospitalization treatment in our hospital from January 2013 to December 2014. And the results of the two sets were analyzed comparatively. Results The re-sults showed that there were 168 cases of positive MP-DNA, accounting for 10.6%, whereas there were 459 cases of posi-tive MP-IgM, accounting for 28.9%, the difference was significant (P<0.05). Conclusion Both real-time FQ-PCR and ELISA serological methods have their own advantages in the diagnosis of MP infection in children. But the false positive rate of FQ-PCR is lower than that of ELISA serological method, so FQ-PCR is more suitable for the early diagnosis of MP infection in children, but using both methods can improve the accuracy of clinical diagnosis.%目的：探讨实时荧光定量PCR与ELISA血清学两种方法学在诊断儿童肺炎支原体(Mycoplasma pneumoniae, MP)感染中所具有的临床诊断价值。方法对2013年1月-2014年12月在该院儿科门诊和住院治疗并怀疑MP感染的婴幼儿及儿童，均常规采集咽拭子或痰标本和EDTA抗凝血2 mL的标本共计1590例，分别用于荧光定量PCR法MP-DNA和ELISA法MP-IgM抗体的检测，并对两组实验结果进行比较分析。结果结果显示MP-DNA阳性共计168例，占10.6%；而MP-IgM阳性共计459例,占28.9%，两者差异有统计学意义(P<0.05)。结论实时荧光定量PCR法与ELISA血清学方法对诊断儿童MP感染有着各自的优势，但PCR法的假阳性率低于ELISA血清学方法，更适合用于儿童肺炎支原体感染的早期诊断，若两种方法同时进行，则可以提高临床诊断的准确性。