A single-chamber, membrane-less and floating-cathode microbial fuel cell (MFC) was established. The effects of operating characteristics on the microbial fuel cell using sludge (SMFC) were studied. Compared to the SMFC without churning, the maximum output power density of SMFC with churning was increased from 45.94mW/m2 to 124.03mW/m2(1300r/min) and 136.5mW/m2(2600 r/min), respectively. This may be attributed to the improvement of the material transmission in SMFC. The influence of temperature on the electrogenesis characteristic of SMFC was generally distinct. However, the power output did not change significantly in the certain temperature ranges (such as 20～25 ℃; 30-40℃; 45～50℃). Electrogenic bacteria had appropriate temperature ranges, and different electrogenic bacteria grew at different temperatures. Compared to the original sludge, the power density of SMFC using sludge undergone microwave treatment or using the supernatant fluid of microwave pretreated sludge was increased rapidly. This was because the microbial competition in the sludge had an important role in SMFC electric efficiency. The increasing of cathodic area could reduce SMFC cathodic potential and internal resistance, which resulted in the increasing of the power density.%实验采用单室无膜悬浮阴极微生物燃料电池(MFC),考察了运行特性对污泥为燃料的MFC(SMFC)的影响.研究表明,相对于未搅拌情况,搅拌时SMFC最大输出功率由45.94mW/m2分别增加到124.03mW/m2(1300r/min)和136.5mW/m2(2600 r/min),主要是由于搅拌有利于改善SMFC内物质的传递.温度对SMFC的产电特性影响较明显,但在一定区间内(如20～25℃；30～40℃；45～50℃)变化不明显,说明产电微生物有一定的温度适应范围,这也可能是在不同温度下产电微生物不同导致.相对于采用未经处理的剩余污泥为燃料,微波处理后的污泥和微波处理过滤后的上清液做燃料时SMFC输出功率迅速增加,这主要是由于污泥中的微生物竞争作用引起.阴极面积的增加有利于降低阴极电势,降低SMFC内阻,从而促进功率密度的增加.
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