Fermented Lysate of Lactiplantibacillus Isolated From Green Tea Leaves Protects Keratinocytes Against Stress Hormone and Staphylococcus Aureus

摘要

Psychological stress can impair epidermal barrier function by inhibiting the proliferation and differentiation of keratinocytes.In this study,the effect of stress hormone on skin microorganisms was confirmed through an in vitro experiment.Cortisol,a typical stress hormone,inhibited the growth of skin microbes,especially Staphylococcus epidermidis,which is a commensal skin microbe.And cortisol enhanced the adhesion of the pathogenic bacterium Staphylococcus aureus to keratinocytes.The fermented lysate of Lactiplantibacillus isolated from green tea leaves(LFL)affected the growth of skin microbes in the opposite manner to cortisol,and increased the expression of a keratinocyte differentiation marker that was suppressed by cortisol and S.aureus.Moreover,LFL inhibited the adhesion of S.aureus to keratinocytes.The modulating effect of LFL on the growth and adhesion of skin microbes was unaffected by the presence of cortisol.LFL also alleviated cell damage in reconstructed human epidermis caused by S.aureus.These results suggest that LFL may be useful as a cosmetic ingredient capable of controlling skin microbiome balance and protecting skin health against psychological stress.