猪伪狂犬病病毒疫苗株与野毒株鉴别PCR检测方法的建立及初步应用

摘要

本试验根据疫苗株缺失gE基因、野毒株和疫苗株均有gB基因的特点,设计两对引物,建立了PCR方法,使用该方法分别对PRV、PCV2、PPV、CSFV、PRRSV进行PCR检测,结果只能从PRV Fa野毒株DNA提取物中扩增出354、237 bp的核苷酸片段,从PRV Bartha-K61疫苗株扩增出237 bp的片段,其他病毒均未扩出条带,达到了区分疫苗株与野毒株的目的.同时,应用该方法对吉林省各大猪场进行了临床病料检测,结果表明,在采集的205份病料中有32份检测为猪伪狂犬病病毒野毒感染,阳性率为15.6％.%On the base of Ge and Gb gene design two primers to distinguish vaccine strains and wild strains. Using this PCR method to detect PRV, PCV2, PPV, PEDV, PRRSV and only PRV could be detected. Using this method to make etiology survey in Jilin province, 32 cases were detected as pseudorabies rabies virus infection in 205 cases,the positive rate was 15. 6%.