广西副猪嗜血杆菌病流行病学调查

摘要

Bacterial isolation was carried out from 86 samples of pigs with suspected multiple serositis and arthritis from 60 pig farms in Nanning,Guilin and Yulin,Guangxi province during May 2009 to November 2010. 26 strains of Hemophilus parasuis (H. Parasuis) were identified by bacterial morphological and cultural characteristics, biochemical characteristics as well as PCR. Isolation rate was 30. 2%. Serological typing,animal challenge and drug sensitive test were performed on the 26 strains of H. Parasuis. The results showed that 26 strains of H. Parasuis were composed of 5 strains of serotype 4,3 strains of sero-type 5,1 strains of serotype 11,13,14,15,respectively,and 12 strains of unknown serotype. Serotype 13,14 and 5 strains of unknown serotype could cause mouse death; however, pathogenicities of other strains were weak. Drug sensitive test indicated that 70% strains of H. Parasuis were high sensitive to Enrofloxacin and Florfenicol, but some strains of H. Parasuis were resistant to other drugs. Our investigation results provided an epidemiological basis for prevention and control of H. Parasuis infection in Guangxi.%本研究于2009年5月至2010年11月调查了广西南宁、桂林、玉林、钦州4个市60个猪场发生副猪嗜血杆菌病的情况.采集病猪组织样品共86份进行副猪嗜血杆菌分离；对疑似菌株进行形态学观察、培养特性、生化特性和PCR鉴定；最终分离鉴定到26株副猪嗜血杆菌,分离率为30.2％；对分离菌株进行血清型鉴定、致病性和药敏试验.结果表明26株分离株中血清4型有5株,5型3株,9、11、13、14、15型各l株,有1株与2、9、10、11型血清均有凝集,其余12株未能鉴定出血清型.血清型5、13、14菌株和5个未能定型的菌株能引起小白鼠全部死亡,其他菌株对小白鼠致病性不强.药敏试验结果表明70％以上的菌株除对恩诺沙星和氟苯尼考高度敏感外,对其他药物敏感性不高.本调查结果将对广西副猪嗜血杆菌病的防治提供指导.