肉碱棕榈酰转移酶基因在绵羊和山羊不同肌肉组织中的表达分析

摘要

采用实时荧光定量PCR方法比较分析了肉碱棕榈酰转移酶(carnitine palmitoyl transferase,CPT)基因在绵羊和山羊不同组织中的表达差异.研究结果表明,CPT1A mRNA在绵羊肝脏、脾脏中表达明显高于CPT1B、CPT2,CPT1B mRNA 在绵羊腹外斜肌中的表达明显高于CPT1A、CPT2.CPT1A mRNA在山羊脾脏中表达明显高于CPT1B、CPT2 mRNA在山羊脾脏中的表达,CPT1B mRNA在山羊后腿股二头肌、腹外斜肌中的表达明显高于CPT1A、CPT2 mRNA在山羊后腿股二头肌中的表达.CPT1A mRNA在绵羊肝脏中的表达显著高于山羊中CPT1A mRNA的表达(P＜0.05).CPT1A、CPT1B、CPT2基因在绵羊心脏、脾脏、腰大肌中的表达显著高于在山羊中的表达(P＜0.05),CPT1A、CPT1B、CPT2基因在山羊后腿股二头肌中的表达显著高于在绵羊中的表达(P＜0.05).CPT1A、CPT1B、CPT2基因在绵羊、山羊各组织中的表达存在差异,可能与各基因表达模式、肌肉组织纤维类型、脂肪沉积含量不同等因素有关.%The author performed the comparative analysis of carnitine palmitoyl transferase(CPT)gene in different tissues from sheep and goats by quantification PCR. The results showed that CPT1A mRNA expression in sheep liver and spleen was higher than CPT1B and CPT2 expression in sheep liver and spleen. Expression of CPT1B mRNA in the external oblique muscle of sheep was higher than CPT1A, CPT2 mRNA expression in the external oblique muscle of sheep. CPT1A mRNA expressed in goat spleen was higher than the expression of CPT1B and CPT2 mRNA in goat spleen. CPT1B mRNA in goats hind legs, biceps femoris and external oblique musclc were higher than CPT1A, CPT2 mRNA in goat hind shares two expression in the biceps. CPT1A mRNA expression in the liver of sheep was significantly higher than the expression of CPT1A mRNA in goats (P<0. 05). CPT1A, CPT1B, CPT2 gene expression in sheep heart, spleen, lumbar major muscle was significantly higher than the expression in goat(P<0. 05) , the CPT1A, CPT1B, CPT2 gene expression in the hind legs in goat was significant higher than the amount of expression in sheep(P<0. 05). CPT family gene expression in the organization of sheep, goat differences might be associated with each gene expression patterns in different types of muscle tissue fiber content of fat deposition and other factors.