西南矮马肌肉生长抑制素基因启动子区PCR-RFLP多态性检测

摘要

In this present study, promoter region of myostation(MSTN) gene of Guizhou pony, Debao pony and Ningqiang pony were analyzed by PCR-RFLP. The results indicated that there was one Ssp I digestion site in the 204 bp amplified fragment. An T→C mutation at the site 156 bp of this gene by DNA sequencing which produced two sorts of genotypes, AA and AB, however, BB genotype was not founded in this study. The genotype frequency of AB in Guizhou pony and Ningqiang pony were higher than AA, while AA genotype was preponderant in Debao pony. The allele frequency of A were higher than B in all these three pony breeds. Guizhou pony and Debao pony were at Hardy-Weinberg equilibrium (P>0. 05) , but Ningqiang pony was significantly different (P<0. 05).%利用PCR-RFLP技术对贵州矮马、德保矮马和宁强矮马3个品种的45个个体的肌肉生长抑制素(myostatin,MSTN)基因的启动子区进行多态性分析.结果表明,大小为204 bp的扩增片段经限制性内切酶Ssp I酶切后,不同基因型经过测序发现156T→C突变,产生AA和AB两种基因型,没有发现BB基因型.贵州矮马和宁强矮马中AB基因型频率大于AA基因型,德保矮马中AA基因型占优势,在3种矮马品种A等位基因频率均大于B等位基因.卡方检测结果发现,贵州和德保矮马在该酶切位点处于平衡状态(P＞0.05),而宁强矮马在该位点分布差异显著(P＜0.05).