ACVR1基因对京海黄鸡生长和繁殖性状的遗传效应研究

摘要

In this study, Jinghai Yellow chicken was used to analyze ACVR1 gene polymorphisms by PCR-SSCP and DNA sequencing method. As a result, there was one mutation site(G20190A) in Jinghai Yellow chicken. Three genotypes were de-tected(AA, AB, BB), and the frequencies of allele A and B were 0. 9075 and 0. 0925. For the reproductive performance, BB genotype in open weight was significantly higher than AA genotype (P<0. 01), however AA genotype in 300 days of age weight was significantly higher than BB genotype (P<0. 05). It implied that ACVRl gene might have a fixed effect on growth and reproductive effect in Jinghai Yellow chicken.%以京海黄鸡为材料,采用PCR-SSCP和DNA测序技术对ACVR1基因外显子多态性进行研究.结果表明,ACVR1基因20190 bp处发生G→A突变,检测到AA、AB和BB 3种基因型.A等位基因频率为0.9075,B等位基因频率为0.0925.基因型与生长和繁殖性状的关联性分析发现,BB基因型鸡的开产体重极显著高于AA基因型鸡(P＜0.01),AA基因型鸡300日龄体重显著高于BB基因型鸡(P＜0.05).由此初步推断,ACVR1基因可能对京海黄鸡的产蛋和发育有一定的影响,A为体重的优势基因,B为产蛋的优势基因.