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策勒黑羊BMPR-IB基因多态性与产羔数的相关性研究

         

摘要

The polymorphism of BMPR-IB gene which was one of the candidate genes on the fecundity of Cele black sheep was analyzed by PCR-RFLP method. The relationship between the polymorphism of BMPR-IB gene and litter size was studied. The average litter size of BB,B+ and ++ were 3. 00, 2. 66 and 1. 98 respectively in 100 Cele black sheep which were selected randomly. The litter size of BB and B+ were significantly higher more than ++ (P<0. 01), but there was no significant difference between BB and B+(P>0. 05). These results indicated that the litter size of Cele black sheep was affected by B allele significantly and it could be used for the selection in Cele black sheep as one of the molecular genetic markers.%以BMPR-IB基因为策勒黑羊多羔性状的候选基因,采用PCR-RFLP方法对策勒黑羊BMPR-IB基因的多态性进行分析,研究其与产羔数的关系.在随机选取的100只策勒黑羊中BB、B+和++基因型对应的每胎产羔数分别为3.00、2.66和1.98只,BB基因型与B+基因型产羔数极显著高于++基因型(P<0.01),BB基因型与B+基因型之间产羔数差异不显著(P>0.05).因此,在策勒黑羊中B等位基因对产羔数有显著影响,可以作为分子遗传标记之一用于对策勒黑羊产羔数的选择.

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