取代基对黄酮类化合物流感病毒神经氨酸酶抑制活性的影响

摘要

本试验旨在研究取代基对黄酮类化合物流感病毒神经氨酸酶(neuraminidase,NA)抑制活性的影响,为黄酮类流感病毒神经氨酸酶抑制剂的研发提供理论依据.应用流感病毒神经氨酸酶活性抑制试验评价了4种黄酮类化合物对NA的抑制活性.芹菜素-7-葡萄糖苷、芹菜素具有H9N2亚型流感病毒NA抑制活性,蒙花苷、汉黄芩苷无H9N2亚型流感病毒NA抑制活性.4’位的取代羟基对于黄酮类化合物的NA抑制活性不可或缺,7位的糖苷键能增强该类化合物的NA抑制活性.%The substituent effects on neuraminidase (NA) inhibitory effect of flavonoids have been studied and could be used to provide experimental data for the exploitation of flavonoids as NA inhibitors. An assay method for evaluating the inhibitory activity of compounds on influenza viral NA was established and was used to evaluate the inhibitory effects of four flavone derivatives. Apigenin-7-glucoside and apigenin possessed inhibitory activity on H9N2 influenza viral Nas. Linarin and wogono-side did not possess inhibitory activity on H9N2 influenza viral Nas. The 4'-OH were essential for NA inhibitory effects of flavonoids and the 7-glycosidic bond could increase NA inhibition of flavonoids.