不同生殖周期阶段对犬卵巢卵母细胞体外发育的影响

摘要

试验以屠宰场犬卵巢为材料,研究了不同生殖周期阶段(卵泡期、黄体期和乏情期)对犬卵母细胞体外发育的影响.结果表明,从卵泡期卵巢采集A类COCs的数量为22.67±11.02个,显著高于黄体期10.67±5.51个和乏情期7.25±4.92个(P＜0.05);体外培养48 h时,卵泡期、黄体期和乏情期卵母细胞达到M Ⅰ-MⅡ期比率分别为19.2％±15.3％、26.8％±21.1％、9.8％±10.4％,卵泡期与黄体期之间差异不显著(P＞0.05),但两者均显著高于乏情期(P＜0.05);体外培养72 h时,卵泡期、黄体期和乏情期卵母细胞达到MⅠ -MⅡ期比率分别为25.2％±13.5％、6.6％±6.7％、9.2％±5.5％,卵泡期显著高于黄体期和乏情期(P＜0.05).同时,随体外培养时间延长,不同生殖周期阶段犬卵母细胞达到MⅠ-MⅡ期比率存在不同变化.说明不同生殖周期阶段对犬卵巢卵母细胞体外发育有一定影响,且与培养时间有关.%Canine ovaries collected from slaughterhouse were used, the effects of the different reproductive stage (follicular stage, corpus luteum stage and anestrus stage ) on IVM of canine oocytes were studied. The results showed that the number of A level COCs from follicular stage was 22. 67±11. 02, significant higher than other groups (P<0. 05). After culture for 48 h, the percentage of oocytes developmented to M Ⅰ -M Ⅱ at various stages were 19. 2% ± 15. 3% , 26. 8% ± 21. 1% , 9. 8% ± 10. 4%. There was no significant difference between follicular stage and corpus luteum stage(P>0. 05), but both were significantly higher than anestrus stage (P<0. 05). After culture for 72 h, the percentage of oocytes developmented to M Ⅰ -MⅡ at various stages were 25. 2 % ± 13. 5 % , 6. 6 % ± 6. 7 % and 9. 2%±5. 5% , follicular stage significantly higher than other groups (P<0. 05). It was found that different reproductive cycle stages of canine ovaries oocytes had certain influence on IVM, but also related with the culture time.