本研究根据GenBank中登录的剂量敏感的翼状螺旋/叉头转录因子2(forkhead transcription factor 2,FOXL2)设计引物,构建包含FOXL2基因cDNA片段的质粒,作为中国荷斯坦牛FOXL2基因mRNA定量检测的标准品,建立了FOXL2基因mRNA表达实时荧光定量PCR检测方法.结果表明,该方法特异性好,检测灵敏度达101拷贝,线性范围为101～106拷贝,阈值循环数(Ct)与PCR体系中起始模板量的对数值之间有着良好的线性关系(r=0.998689),扩增效率高(E=99.62％),可以作为检测牛FOXL2基因mRNA定量检测方法.%The primer was designed based on forkhead transcription factor 2CFOXL2), according to the sequence which was submitted on GenBank. A recombinant plasmid contained cDNA fragment of FOXL2 gene was constructed and used for the standard substance for quantitative detection of mRNA of the FOXL2 gene of Holstein, a Real-time PCR was developed for detection of FOXL2 gene. The results revealed this method had a good specificity, and the sensitivity was up to 101 , range from 101 to 106. The cutoff value Ct had a good linear correlation with PCR samples. This method can detecte FOXL2 gene in bovine quantitatively.
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