为了研究B亚群禽白血病病毒(ALV-B)的半数细胞培养物感染量(TCID50)与p27抗原的酶联免疫吸附试验(ELISA)的S/P值之间的相关性,本试验将ALV-B SDAU09C2株接种鸡胚成纤维细胞(CEF细胞),换维持液后连续10 d取样,检测10d的TCID50值与p27抗原的S/P值之间的相关性；同时,将该毒株在DF-1细胞系上传代至20代,取其中的第1、5、10、15和20代分别进行TCID50滴度的测定和p27抗原检测.结果表明,在CEF细胞上接种的ALV-B SDAU09C2株连续10 d的TCID50值与p27抗原之间存在显著的正相关(r=0.94002;P＜0.0001)；在DF-1细胞系上传的不同代数之间也呈显著正相关(r=0.96449;P=0.0080).由此可推测ALV-B的TCID50与p27抗原呈显著正相关,可以用ELISA法测得的p27抗原的S/P值来估测病毒的TCID50值.%To study the correlation between 50% tissue-culture infective dose (TCID50) value and p27 antigen's S/P value of subgroup B avian leukosis virus and discuss their significance, chicken embryo fibroblast (CEF) cells were inoculated with subgroup B avian leukosis virus strain SDAU09C2 and samples were taken continuously for ten days after changing maintenance media. The correlations between TCID50 value and p27 antigen's S/P value of ten days were then detected. Simultaneously, DF-1 cells were inoculated with it and passed to 20 generations. Samples taken from 1st, 5th, 10th, 15th and 20th generation were received the TCID50 titer determination and the p27 antigen examination separately. A significant Pearson correlation was found between them in CEF cells (r=0. 85277;P<0. 0001) and in DF-1 cells 0=0. 93000; P = 0. 0220). This study provided an important parameter for predicting TCID50 by detecting the p27 antigen's S/P value.
展开▼
