牦牛源牛链球菌的分离鉴定及部分生物学特性研究

摘要

本研究旨在从腹泻牦牛粪便中分离鉴定牛链球菌,并分析其溶血性、对小鼠的致病性及对抗菌药物的敏感性。将川西北阿坝州45份腹泻牦牛粪便于血平板上划线,37℃、5% CO2培养24 h 分离细菌,经16S rRNA 序列扩增测序和系统发育分析鉴定出14株牛链球菌,其中8株巴黎链球菌,6株解没食子酸链球菌巴氏亚种；9株呈α溶血,5株呈β溶血。分离鉴定的牦牛源巴黎链球菌和解没食子酸链球菌巴氏亚种能引起试验小鼠的轻度腹泻；药敏试验结果显示分离菌株对青霉素、头孢噻肟、万古霉素、乙酰螺旋霉素、环丙沙星、利福平、替考拉宁、氨苄西林和庆大霉素共9种抗生素高度敏感,对链霉素、卡那霉素、林可霉素、红霉素、四环素和克林霉素耐药率较高。本研究阐明了牦牛源链球菌的部分生物学特性,为该病的防控奠定了基础。%The objective of this study was to isolate and identify Streptococcus bovis from yak,and detect their hemolytic,pathogenicity and antimicrobial susceptibility.45 fecal samples collected from the diarrheal yaks in Aba state in Northwest Sichuan province were used to isolate Strepto-coccus bovis with sheep blood culture media under 37 ℃ and 5% CO2 condition cultured for 24 h. The isolated bacteria were identified by 1 6S rRNA amplification and sequencing.Total 14 Strep-tococcus bovis were identified,among which,8 Streptococcus lutetiensis strains and 6 Streptococ-cus gallolyticus strains.9 Streptococcus bovis strains showed α hemolytic,and 5 Streptococcus bovis showed β hemolytic.Both Streptococcus lutetiensis and Streptococcus gallolyticus caused the experimental mice mild diarrhea.The isolated 14 Streptococcus bovis were sensitive to penicil-lin,cefotaxime,vacomycin,acetylspiramycin,ciprofloxacin,rifampicin,teicoplanin,ampicillin and gentamicin,and highly tolerant to streptomycin,kanamycin,lincomycin,erythrocin,tetra-cycline and clindamycin.This study illustrated the biological characteristics of the isolated Strep-tococcus bovis from yak,which made the basis for the prevention and control of diseases caused by Streptococcus bovis .