为了解中国优良地方猪种梅花猪胃肠道内分泌细胞的分布与形态特征,本试验选取6头30日龄断奶健康梅花仔猪,在相同条件下饲养至65日龄,所有仔猪饲喂相同日粮,自由采食饮水.试验结束时,收集仔猪胃肠道各段组织样品,应用免疫组织化学方法观察3种胃肠道内分泌激素5-羟色胺(5-HT)、胃饥饿素(ghrelin)和胰高血糖素样肽-1(GLP-1)免疫阳性细胞的分布及形态特征,并对阳性细胞密度进行分析.结果显示,梅花猪的肠道不同部位(十二指肠、空肠、回肠和结肠)均存在5-HT阳性细胞,主要位于肠上皮组织中,呈锥形、圆形、梭形等形态,可分为开放型和闭合型,其中十二指阳性细胞肠密度显著高于其他肠段(P<0.05);梅花猪的胃肠道不同部位均有Gh-relin阳性细胞,阳性细胞密度也是随胃肠道走向递减,胃腺体部密度最高;梅花猪的肠道不同部位均有GLP-1阳性细胞,阳性细胞密度沿肠道走向呈抛物线分布,其中回肠数量最多.本试验结果为进一步探究梅花猪胃肠道生理奠定了基础.%In order to analyze the distributions and densities of endocrine cells in the gastrointesti-nal tract of Meihua pig (an elite native breed of Guangdong province,China),6 healthy piglets weaned at 30 days of age were fed with the same diet till 65 days of age.At 65 days of age,differ-ent sections of the gastrointestinal tract of each piglet were collected,and the features and densi-ties of 5-hydroxytryptamine (5-HT),ghrelin and glucagon-like peptide-1 (GLP-1 )immunoposi-tive cells were detected by immunohistochemistry.The results showed that the 5-HT positive cells distributed at the epithelial layer throughout the intestinal tract (duodenum,jejunum,ileum and colon)with a variety of features (e.g.cone,round,spindle),and they could be classified in-to either open-shape or closed-shape type,the density of 5-HT positive cells in duodenum was highest;The ghrelin positive cells were located along the gastrointestinal tract (gastric glands, pylorus,duodenum and jejunum)with both open-shape and closed shape,and its density de-creased along the tract with most cells in gastric glands;The GLP-1 positive cells were observedalong the intestinal tract,but its density increased from duodenum to ileum and then decreased in colon.This study might provide supporting data for further study of the gastrointestinal physiol-ogy of Meihua pig.
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