N-羟甲基蛋氨酸钙水平对泌乳期奶牛血清含氮物和游离氨基酸的影响

摘要

为研究N-羟甲基蛋氨酸钙(N-hydroxymethyl methionine calcium,N-HMM-Ca)对荷斯坦奶牛血清含氮物和游离氨基酸的影响,试验选用胎次、产奶量和泌乳天数相近的30头健康荷斯坦奶牛,随机分为5组.对照组饲喂基础日粮,试验组分别在基础日粮中添加0.15%、0.30%、0.75%和1.50% N-HMM-Ca,预试期14 d,正试期42 d.结果显示:①日粮中补充N-HMM-Ca对奶牛血清中总蛋白、白蛋白、球蛋白含量无显著影响(P>0.05).第14天,1.5%组血清肌酐浓度显著低于对照组(P<0.05);第42天,1.5%组尿素氮浓度显著低于对照组(P<0.05),而0.75%组尿酸浓度极显著高于其他组(P<0.01).②第14天,0.15%组血清中谷氨酸、丝氨酸、精氨酸、亮氨酸、异亮氨酸、赖氨酸及总游离氨基酸浓度最高,与对照组相比差异显著或极显著(P<0.05;P<0.01);试验第42天,0.15%组血清中精氨酸、胱氨酸及总游离氨基酸浓度最高.0.30%组血清中谷氨酸、丝氨酸、酪氨酸、蛋氨酸、缬氨酸、赖氨酸浓度最高,与对照组相比差异显著或极显著(P<0.05;P<0.01).综上所述,在基础日粮中添加0.15%、0.30% N-HMM-Ca对泌乳期奶牛血清含氮物含量无显著影响(P>0.05),且添加0.15% N-HMM-Ca能够显著提高泌乳期奶牛血清游离氨基酸含量,综合考虑,N-HMM-Ca的添加量以0.15%为宜.%To study the effects of N-hydroxymethyl methionine calcium (N-HMM-Ca) supplementation levels on serum nitrogenous compounds and free amino acids of lactating dairy cows.30 lactating Holstein dairy cows with similar parities,milk yield and days in milk were randomly assigned into 5 groups.The control group was fed with basal diet,test groups were fed with basal diet supplemented with 0.15%,0.30%,0.75% and 1.50% N-HMM-Ca,respectively.The experiment was lasted for 56 d,including 14 d of adaptation period and 42 d of experimental period.The results showed as follows:①Comparing with control group,the total protein,albumin and globulin in experimental groups were not significantly affected (P>0.05).On the 14th day,serum creatinine concentration in 1.5% level group was significantly lower than control group (P<0.05);On the 42th day,urea nitrogen concentration in 1.5% level group was significantly lower than control group (P<0.05),while the concentration of uric acid in 0.75% level group was extremely significantly higher than that in other groups (P<0.01).②On the 14th day,the concentration of serum Glu,Ser,Arg,Leu,Ile,Lys and total free amino acids in 0.15% level group was highest,which were significantly or extremely significantly higher than control group (P<0.05;P<0.01).On the 42th day,serum Arg,Cys and the total free amino acids in 0.15% level group were the highest.And serum Glu,Ser,Tyr,Met,Val and Lys in 0.30% level group were highest,which were significantly or extremely significantly higher than the control group (P<0.05;P<0.01).In summary, adding 0.15% or 0.30% N-HMM-Ca on diet of lactating dairy had no significant effect on serum nitrogen content (P>0.05),but adding 0.15% N-HMM-Ca could increase the concentration of serum free amino acids.Considering all the facts,the recommended amount of N-HMM-Ca on diet of lactating dairy cows was 0.15%.