卵母细胞核因子促进核移植四倍体胚胎早期发育研究

摘要

研究旨在探讨猪卵母细胞核因子在重编程过程中发挥的作用。将体细胞引入未去核的 MⅡ期卵母细胞中，构建体细胞核与卵母细胞核共存的核移植四倍体胚胎。通过分析核移植四倍体胚胎的早期发育情况探讨卵母细胞核因子对核移植四倍体胚胎早期发育的影响。结果显示，核移植四倍体胚胎、孤雌二倍体胚胎及孤雌单倍体胚胎这3组胚胎的卵裂率极显著高于核移植二倍体胚胎（P ＜0．01），且核移植四倍体囊胚率及总细胞数也极显著高于核移植二倍体囊胚（P ＜0．01）。与通过标准核移植程序构建的核移植二倍体胚胎相比，核移植四倍体胚胎具有更强的发育能力。本研究建立了一个体细胞核与完整卵母细胞核因子物质共存的四倍体胚胎模型，有助于研究供体核与卵母细胞核之间的联系，为研究核因子在重编程过程中发挥的作用提供了平台。%The study was aimed to investigate the role of porcine oocyte nuclear factors during re-programming.Somatic cell nuclei was introduced into intact MⅡ oocytes to establish tetraploid somatic cell nuclear transfer (SCNT)embryos containing both somatic nuclei and oocyte nuclei. And then the influence of the oocyte nucleus on tetraploid SCNT embryo development was exam-ined by assessing characteristics including cleavage rate and blastocyst rate.The results showed that the cleavage rate of tetraploid SCNT embryos,diploid parthenogenetic embryos and haploid parthenogenetic embryos was extremely significantly higher than that of standard diploid SCNT embryos (P <0.01).The blastocyst rate and the total number of cells in tetraploid SCNT embry-os were extremely significantly higher than that of standard diploid SCNT embryos (P <0.01 ). Overall,tetraploid SCNT embryos had a higher developmental competence than standard diploid SCNT embryos.In conclusion,the embryonic model was established in which a fetal fibroblast nucleus and an oocyte M Ⅱ plate coexist.Tetraploid SCNT represented a new research platform that was potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.