牛血红蛋白源抗菌肽P3及其类似肽的生物信息学分析

摘要

To provide basic information for researching the biological function of antimicrobial peptide P3 from bovine hemoglobin and its analogs (JH-0,JH-1,JH-2 and JH-3),tools on line were utilized to analyze some bioinformatic features of these five peptides.The results showed that the isoelectric point of these five peptides were all more than 8.00.All of these five peptides had positive charge,no transmembrane domain,no signal peptide sequence,no glycosylation sites,were hydrophobins and located at extracellular.The secondary structure of peptide P3 and JH-2 had α-helix andβ-sheet,JH-3 was all α-helix,while JH-0 and JH-1 were coil.Peptide P3, JH-2 and JH-3 had a glycosylation site at the thirteenth site of amino acid (Thr,T),respective-ly.Based on these results,we conjectured that antimicrobial peptide P3 and its analogs could kill bacteria through the interaction with cell wall or cell membrane.%为研究牛血红蛋白源抗菌肽 P3及其类似肽（JH-0、JH-1、JH-2、JH-3）的生物学功能，本研究利用在线软件分析了抗菌肽 P3及其类似肽的生物信息学特性，发现这5个抗菌肽均带正电荷，等电点均大于8．00，均为疏水蛋白，定位于细胞外，不含有跨膜区、信号肽序列和糖基化位点，P3和 JH-2的二级结构为α-螺旋＋β-折叠，JH-3为100％的α-螺旋，JH-0和 JH-1为无规则卷曲结构，P3、JH-2和 JH-3在第13位氨基酸即苏氨酸（Thr，T）有一个糖基化位点。根据分析结果推测抗菌肽 P3及其类似肽可作用于细胞壁或细胞膜，从而使菌体死亡。