BHK-21细胞生物反应器悬浮培养参数研究

摘要

Suspension culture parameters of BHK- 21 were studied by using domestic BC- 7L bioreactor. The most suitable suspension culture parameters of BHK- 21 cells were determined by adjusting the factors respec-tively. The most suitable parameters is 5%serum concentration, pH 7.40, 50%dissolved oxygen and 100 r/min motor.%使用国产BC-7L生物反应器对BHK-21细胞悬浮培养参数进行了初步研究。通过对培养液血清含量、pH、溶氧和搅拌转速逐一进行优化研究，最终确定了BHK-21细胞悬浮培养的最佳参数为：血清浓度为5%，pH为7.40、DO为50%，搅拌转速为100 r/min。