无菌采取青海省部分地区藏獒血样和直肠深部粪便各60份，应用酶联免疫吸附试验（ELISA）和犬细小病毒检测试纸对藏獒细小病毒感染进行了血清学调查和病原检测。结果显示，在被检的60份藏獒血清样品中，共检出阳性血清6份，平均阳性率为10%（6/60）。犬细小病毒检测试纸检出阳性粪便5份，平均检出率为8.33%（5/60）。两种检测方法阳性符合率为83.33%，经统计分析，其差异不显著（ P>0.05），表明两种方法在兽医临床中均可应用于细小病毒检测，虽然试纸法简便、特异、快速、结果直观，便于在基层推广使用，但为提高实验结果的准确性最好将两种方法结合应用。%60 Tibetan mastiff serum samples and 60 feces of deep-seated intestinum rectum were collected by asepsis in part areas of Qinghai province,for detection of antibodies against canine parvovirus respectively by ELISA and CPV test paper. The results showed that 6 serum samples were detected positive for CPV by ELISA, with a positive rate of 10%(6/60)and 5 fecas samples were detected positive for CPV antigen with a positive rate of 8.33%(5/60). Statistic analysis showed that there was no significant difference between the two methods (P>0.05) and both the methods could be applied to CPV detection in veterinary clinic. The test paper method is simple, specific, quick, direct and easy in operation at grassroots level,however in order to improve the accuracy of the experiment result, it is better to combine the two methods in practice.
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