川西北牦牛布鲁氏菌病血清流行病学调查

摘要

The indirect ELISA(iELISA),the Rose bengal plate test(RBPT)and standard tube agglutination test(SAT), were used to investigate the serological prevalence of bovine brucellosis in yak in regions ordering northwestern Sichuan province of China. A total of 1070 serum samples collected from 8 counties in the grassland area in northwest Sichuan province of China,were examined to investigate the brucellosis sero-prevalence by the three methods. Serological evi-dence indicated that the apparent prevalence of brucellosis in yaks was 25.4%(272/1070) detected by iELISA,12.7%(136/1070)by RBPT,and 9.1%(97/1070) by STAT,suggesting that yak brucellosis existed in all the 8 counties under in the study. The relative sensitivity and speciifcity of RBPT were 50.0%and 100%,those of STAT were 35.7%and 100%respectively,compared with iELISA. The results shown that iELISA was the most sensitive method.%同时采用间接ELISA、虎红平板凝集试验和试管凝集试验三种血清学方法调查川西北牦牛布鲁氏菌病血清流行情况。对1070份采自川西北阿坝州8个草地县的牦牛血清样品经三种血清学方法检测，间接ELISA检测阳性率为25.4%（272/1070），虎红平板凝集试验检测阳性率为12.7%（136/1070），而试管凝集试验检测阳性率为9.1%（97/1070）。8个县均发现阳性布鲁氏菌病血清。与间接ELISA相比，虎红平板凝集试验的相对敏感性和特异性分别为50%和100%，而试管凝集试验的相对敏感性和特异性分别为35.7%和100%。布鲁氏菌病在川西北地区广泛存在，血清学方法是检测和监测布鲁氏菌病最常用和最简便的方法，其中间接ELISA方法最为敏感。