进口南美白对虾亲虾急性肝胰腺坏死病的检测分析

摘要

Acute hepatopancreatic necrosis disease(AHPND)is an emerging disease causing enormous loss to shrimp aquaculture. The pathogen is a kind of Vibrio parahaemolyticus with plasmid of toxin,but its detailed pathogenic mechanism is still unclear. The primers targeting toxin plasmid were used to detect AHPND from a batch of imported Penaeus vannamei broodstock in this study. The PCR results showed that the same sized band confirmed AHPND was positive. Analysis on the results and processes of performance provided important data for AHPND detection in China.%急性肝胰腺坏死病（AHPND）是一种新发疫病，对对虾养殖业损失巨大。其病原是携带毒素质粒基因的副溶血弧菌，但具体致病机制仍不清楚。本研究利用国外报道的毒素质粒基因特异性引物，对一批进境南美白对虾亲虾进行了AHPND检测。因其PCR的扩增结果与国外所报道的基因片段一致，初步认定为AHPND阳性。本文对此次检测的结果和过程进行了分析，为我国AHPND的检测提供了分析数据。