高效液相色谱法同时测定互叶白千层中的槲皮素和山奈酚

摘要

用高效液相色谱法同时测定广西5地互叶白千层中槲皮素和山奈酚含量.样品用盐酸-甲醇(体积比5∶100)混合溶液水浴加热回流提取,C18柱色谱分离,以甲醇-乙腈-0.4％磷酸(体积比21∶21∶58)水溶液为流动相,检测波长370 nm,柱温为室温,流速1 mL/min.测定了不同产地互叶白千层中的槲皮素和山奈酚质量分数,其中邕宁南晓的槲皮素和山奈酚的质量分数最高,分别为5.151和2.530 mg/g,邕宁的槲皮素和山奈酚的质量分数为最低,分别为2.386和1.658 mg/g.%A high-performance liquid chromatography( HPLC) method was developed for simultaneous determination of quercetin and kaempferol in Melaleuca alternifolia ( Maiden et Betehe) Chee 1. The sample was extractd by HCl-MeOH solution(volume ratio 5:100) in water bath, then separated by a C18 column with CH3OH-C2H3N-0. 4% phosphoric acid solution (volume ratio 21 :21 :58) as the mobile phase solution under 370 nm wavelength. The HPLC experiment was performed at room temperature with flow rate 1.0 mL/min. Quercetin and kaempferol contents in M. alternifolia from different area were determined. The result showed that quercetin and kaempferol content in Nanxiao town of Yongning was the highest and that in Yongning was the lowest.