以不同产地人工林中采取的降香黄檀( Dalbergia odorifera T. Chen)木材为研究对象,提取并扩增不同温度处理后的降香黄檀心、边材DNA和ITS片段,分析不同温度处理对降香黄檀木材DNA提取和PCR扩增的影响；对不同产地的降香黄檀木材及其近缘种多裂黄檀( Dalbergia rimosa Roxb)木材的rDNA-ITS序列进行测定和差异分析。结果表明,25℃和65℃热处理后的木材DNA呈不同程度的弥散分布,105℃热处理后的木材DNA降解成250bp以下的小片段；不同温度处理后的降香黄檀木材,仅有25℃处理后的木材ITS片段能够被成功地扩增。降香黄檀和多裂黄檀ITS序列共存在6个变异位点且ITS2区变异大于ITS1区,聚类分析结果可以将降香黄檀及其近缘种多裂黄檀木材区分开来,为利用ITS条形码序列鉴定降香黄檀木材及其常见混伪品提供理论依据。%The wood of Dalbergia odorifera T. Chen originated from different sites of plantations were sampled as materials in this paper. DNA from the sapwood and heartwood of D. odorifera treated with various temperature was extracted and amplified for the internal transcribed space ( ITS) sequences. The effects of various temperature treatments on DNA extraction and amplification for target sequences were analyzed. The rDNA-ITS sequences of D. odorifera from different habitats and D. rimosa Roxb, a closely related species, were sequenced and their divergences were analyzed. The results showed that there were varying extents of diffusion with the DNA extracts from the heat treatment of 25℃ and 65℃, and the DNA from the heat treatment of 105℃ was degraded and smaller than 250bp. The ITS sequences can be amplified successfully from wood after 25℃ heat treatments. The distribution of vriations in ITS2 regions of D. odorifera and D. rimosa was larger than ITS1 regions and there were 6 variable sites in both of ITS regions. D. odorifera and D. rimosa could be distinguished by cluster analysis. Therefore, it could provide theoretical reasons to identify wood of D. odorifera from adulterants by using the rDNA-ITS sequences.
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