高速及高分辨率原子力显微镜用于研究Trichoderma reesei纤维素酶在结晶纤维素上的行为

摘要

介绍了原子力显微镜( AFM)和高速原子力显微镜( HP-AFM)在研究生物大分子动态过程中的优势和Trichoderma reesei纤维素降解酶系的情况。详细综述了高速原子力显微镜和高分辨率原子力显微镜( HR-AFM)应用于研究 Trichoderma reesei 产生的3种主要纤维素酶 Cel7A (TrCel7A),Cel6A(TrCel6A)和Cel7B(TrCel7B)在结晶纤维素上行为所获得的结果。可知,结晶纤维素载入催化结构域和糖苷键的水解是Cel7A运动所必须的；增加结晶纤维素的疏水面积、Cel6A和Cel7A的协同作用能提高结晶纤维素水解速率；Cel7B在起初引起结晶纤维素表面的水解之后引起润涨。可以预期HP-AFM将在阐明纤维素酶在结晶纤维素上的作用机制方面发挥巨大作用。%The advantages of the atomic force microscopy and high speed atomic force microscopy on studying the dynamics of bio-macromolecule and the cellulytic enzyme system of Trichoderma reesei were presented. The results of high speed atomic force microscopy and high resolution atomic force microscopy in investigating the actions of Cel7A(TrCel7A), Cel6A(TrCel6A) and Cel7B(TrCel7B) of Trichoderma reesei on crystalline cellulose were reviewed in detail. The conclusion reveals that the loading of crystalline cellulose into catalytic domain and the hydrolysis of the glucosidic bond are prerequisite for the movement of Cel7A;increasing the hydrophobic area of crystalline cellulose and the combination of Cel6A and Cel7A enhance the hydrolytic rate of crystalline cellulose;Cel7B makes crystalline cellulose swelling. And it is prospective that HP-AFM will play an important role in elucidating the acting mechanism of cellulase on crystalline cellulose.