N-(9-anthracene methyl)-L-tyrosine hydrochloride as a fluorescent subject was synthesized via the reaction of fluorophore 9-anthracene with L-tyrosine. The recognition of resultant fluorescent subject for various anions was examined by means of fluorescence spectro-metry. The optimal molar ratio of the fluorescent subject vs ligand was determined. It was found that molecular recognition interaction occurred between Cr2O27- (5×10-5 mol ●L-1) and the fluorescent subject (1×10-3 mol●L-1, 100 /μL) in methanol solution of pH = 6, by way of the coordination molecular recognition leading to fluorescence quenching at a complexing constant of K - 748 mol●L-1. This means that the molecular recognition subject N-(9-anthracene methyl)-L-tyrosine hydrochloride possesses a higher selectivity to Cr2O27-. Under the same condition, F- showed redshift effect in relation to the ligand; and it was able to determine the optimal mole radio between F~ and fluorescent subject based on fluorescence titration.%利用荧光基团9-蒽醛与L-酪氨酸反应,合成了荧光主体N-(9-蒽甲基)-L-酪氨酸盐酸盐；采用荧光光谱方法检测了荧光主体与不同阴离子之间的识别作用；通过加入不同摩尔比的配体确定了主体与配体之间识别的最佳摩尔配比.结果表明,配置浓度为5×10-5mol·L-1的Cr2O2-7溶液后,用微量进样器加入100μL浓度为1×10-3mol·L-1的主体溶液,在pH=6的甲醇水溶液中进行荧光滴定试验,分子识别主体能与Cr2O2-7分子发生配位识别作用,从而引起荧光猝灭；配位常数K=748 mol·L-1.这表明分子识别主体N-(9-蒽甲基)-L-酪氨酸盐酸盐对Cr2O2-7有较高的选择性；同样条件下F-对分子识别主体发生红移现象,通过滴定试验可以确定F-与主体溶液之间的最佳摩尔配比.
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