高效液相色谱法同时分离检测食品添加剂

摘要

建立了一种同时分离检测没食子酸丙酯(PG)、特丁基对苯二酚(TBHQ)、二丁基羟基甲苯(BHT)和苯甲酸等食品添加剂的高效液相色谱(HPLC)分析方法.通过对流动相、流速、柱温、检测波长等色谱条件的优化,确定了最佳的分离检测条件:甲醇(B)和水(A)(0.1%的乙酸溶液)作为流动相,梯度洗脱:0~6 min,60%B,流速0.3 mL/min;6~9 min,60%~100%B,流速0.6 mL/min,柱温维持在25℃,检测波长280 nm(PG、TBHQ、BHT),230 nm(苯甲酸).在最佳的色谱条件下,方法的线性范围分别为1~200、2~400、2~400、0.5~400 mg/L,检出限(LOD)为0.02~0.06 mg/L,相关系数(R2)均大于0.9994,加标回收率在77.76%~106.27%之间.该方法还成功应用于话梅、薯片、方便面实际样品中食品添加剂的分析检测.%A HPLC method for the analysis of food additives including propyl gallate ( PG) , tert-Bu-tylhydroquinone ( TBHQ) , butylated hydroxytoluene ( BHT) and benzoic acid in foods was developed. The chromatographic conditions with mobile phase, flow rate, column temperature were optimized, and methanol ( B) and water with 0.1% acetic acid ( A) were used as the mobile phase with that the gradi-ent program is 0-6 min, 60%B, the flow rate of 0.3 mL/min;6-9 min, 60%-100%B, the flow rate of 0.6 mL/min. The column temperature was maintained at 25℃. Under the optimized conditions, the linearity range of the method for the analysis of PG, benzoic acid, TBHQ, BHT were 1-200, 2-400, 2-400 and 0.5-400 mg/L with correlation coefficients ( R2 ) with more than 0.9994. The limits of de-tection ( LODs) were in the range of 0.02-0.06 mg/L at a signal-to-noise ratio of 3, the recoveries of these compounds were in the range of 77.76%-106.27%. The developed method was also applied to a-nalysis real samples.