一种阿霉素/NO供体光敏纳米复合物的合成与性质研究

摘要

In this paper, we described the synthesis of a novel light-sensitive nanocomposites that con-sisted of CuS nanocrystal as the core and poly ( N-isopropylacrylamide)-graft-chitosan ( PNIPAM-g-CS) microgel as the shell. The CuS@PNIPAM-g-CS nanocomposites were synthesized by temperature-tunable copolymerization of NIPAM and CS in the presence of CuS nanocrystals. Due to the photother-mal effect of CuS under NIR light ( 980 nm) irradiation, the nanocomposites presented photothermal-sensitive volume shrinkage of PNIPAM-g-CS microgel. After loading of doxorubicin(Dox), the nano-composites were utilized as versatile nanocarriers for photothermal-induced release of Dox. Nitric oxide (NO) photodonors (RBS) were then loaded into nanocomposites to fabricate Dox/RBS dual-loading CuS@PNIPAM-g-CS nanocarriers. Upon visible light (365 nm) irradiation, the RBS could release NO due to the photolysis. Experimental results implied that NIR and visible light respectively triggered re-lease of Dox and NO from nanocarriers. Together with photothermal effect of CuS, the nanocarriers re-alized light-triggered release of dual-drugs and synergistic chem-photothermal therapy to cancer cells.%以硫化铜纳米晶(CuS-NCs)为核心,聚N-异丙基丙烯酰胺接枝壳聚糖(PNIPAM-g-CS)微粒为壳合成一种新型光敏纳米复合材料.在温度的调节下,N-异丙基丙烯酰胺(NIPAM)包覆CuS纳米晶,并接枝壳聚糖(CS),合成CuS杂PNIPAM-g-CS纳米复合材料.CuS在近红外光(980 nm)照射下具有光热效应,导致纳米复合物中PNIPAM-g-CS微粒受热体积收缩.负载阿霉素,这种纳米复合物就可作为光热诱导释放阿霉素的多功能纳米载体.再负载NO光敏供体(RBS),就可制备出阿霉素/RBS双负载的CuS杂PNIPAM-g-CS纳米载体.在可见光(365 nm)照射下,RBS光解释放NO.近红外光和可见光分别触发纳米载体释放阿霉素和NO,加上CuS纳米晶的光热效应,这种纳米载体可实现光触发双药物释放协同光热化疗杀伤肿瘤细胞.