Synthesis and Analytical Application of Bis(2-diazoaminobenzothiazoyl)-benzidine to Determination of Mercury Ion by Fluorimetry

摘要

A new fluorescent reagent bis(2-diazoaminobenzothiazoyl)-benzidine(BDABTB) was synthesized by combining benzothiazole with benzidine and triazene reagent. The product was confirmed by elemental analysis, 1H NMR and FTIR. The excitation and emission wavelengths of BDABTB were 214 and 338 nm in a basic medium, respectively. The fluorescence was quenched by Hg2+ selectively and sensitively. The linear range of Hg2+ was 1.0×10-8―1.0×10-5 mol/L with a detection limit of 5.0×10-10 mol/L. It has been used to determine trace Hg2+ in water sample with satisfactory results.