环氧树脂微流控芯片的表面功能化修饰

摘要

Microfluidic devices are potentially useful for biomedical detection, which sometimes need to be modified on the surface to immobilize active biomolecules. Surface modification of air-plasma-pretreated epoxy resin via near UV-induced graft polymerization with acrylic acid (AAc) was carried out. Specific antibodies were covalently immobilized on the surface of epoxy resin which were used for immunological detection. The modified epoxy resin was characterized by static water contact angle measurement, toluidine blue O staining test, X-ray photoelectron spectroscopy ( XPS), bicinchoninic acid (BCA) test, and cell attachment. The resuits show that the carboxyl groups are grafted onto the surface of epoxy resin, and covalently crosslinked with proteins. The results of cell attachment reveal that the modified epoxy resin could be applied for immunological detection.%对一种新型的环氧树脂材料表面用空气等离子体进行前处理,再通过丙烯酸(Acrylic acid,AAc)紫外诱导接枝聚合在该环氧树脂材料表面引入羧基基团.在此基础上,将这种化学惰性的环氧树脂表面连接上特异的抗体,作为微流控芯片的基底进行免疫检测实验.采用静态接触角、甲苯胺兰染色、X射线光电子能谱(XPS)分析、BCA蛋白定量检测和细胞黏附实验等检测手段对修饰的环氧树脂表面进行了表征.结果表明,使用空气等离子体处理后再经丙烯酸紫外诱导接枝聚合已成功地在环氧树脂表面引入了大量的羧基基团;进一步使用EDC/NHS偶联试剂将蛋白以共价连接的方式连接到疏水的、化学惰性的环氧树脂表面.细胞黏附实验表明,用这种方法修饰的环氧树脂表面可以用于生物医学的免疫检测实验.