多苯并咪唑锰(Ⅱ)配合物的合成及对DNA凝聚的促进作用

摘要

以多苯并咪唑配体1,1,4,7,7-五(2-苯并咪唑甲基)-二乙基三胺( DTPB)为主配体,合成了锰(Ⅱ)配合物[Mn(DTPB) Ac] Ac· 8H2O(1)和[Mn2 (DTPB)( NO3)2(H2O)2][Mn2( DTPB)( NO3)2(H2O)( CH3 OH)]·(NO3)4·5CH3OH·H2O(2),并对其进行了表征.利用紫外-可见吸收光谱和黏度实验研究了配合物1和2与DNA的相互作用,发现这2个配合物均能与DNA结合,并对配合物与DNA作用的机理进行了探讨.利用琼脂糖凝胶电泳和直角光散射(RALS)技术研究了配合物1和2促进DNA凝聚的性质.结果表明,在近中性条件下2个配合物都能促使DNA凝聚.利用透射电子显微镜(TEM)观察了不同凝聚体的形态.%Complexes [ Mn( DTPB)Ac] Ac·8H2O(1) and [ Mn2(DTPB) ( NO3 )2( H2O)2 ] [Mn2(DTPB)·( NO3 )2·( H2O)·( CH,OH) ] ( N03 )4 -5CH3OH -H2O(2) were synthesized via 1,1,4,7,7-pentakis( lff-ben-zimidazol-2-ylmethyl)-l ,4,7-triazaheptane(DTPB) as the main ligand and characterized by X-ray diffraction. The interaction between complexes 1 or 2 and DNA, examined by UV-Vis absorption spectra and viscosity experiments, indicated that the two Mn( II ) complexes have strong affinity for DNA. The interaction mechanism of the complexes with DNA was discussed. These Mn( II ) complexes can promote DNA condensation under neutral conditions, as illustrated by gelose gel electrophoresis and right angle light scattering ( RALS). The morphologies of the DNA condensates were observed by transmission electron microscopy(TEM).