基于表面等离子体子共振成像技术研究苏拉明和顺铂对肝癌细胞生长的抑制作用

摘要

A rapid, label-free and novel evaluation of anti-cancer drug efficacy was proposed via surface plas-mon resonance imaging( SPRi) . A microfluidic chip with micro-posts was designed and fabricated in polydime-thylsiloxane( PDMS) to work as a continuous flow cell. Specific entrapment and detection of tumor cells were accomplished by a homemade SPRi system with the microfluidic chip. On this basis, the inhibitory effects of suramin and cisplatin on the proliferation of HepG2 cells was investigated. Meanwhile, we used auxiliary cell counting kit-8(CCK-8) assay to examine cell viability after drug incubation. The results of the SPRi method showed that both suramin and cisplatin could inhibit the proliferation of HepG2 cells in a dose-dependent and time-dependent manner.%基于表面等离子体子共振成像( SPRi)技术提出了一种实时、非标记的新型抗癌药物药效评估方法。以聚二甲基硅氧烷( PDMS)为材料，制作了包含微柱结构的微流控芯片作为流通反应池，配合自行设计组装的SPRi生物传感器完成肿瘤细胞的特异性捕获及检测，研究了苏拉明和顺铂对肝癌细胞HepG2的生长抑制作用。同时引入辅助验证实验，即采用常规八肽胆囊收缩素(简称CCK-8)法测定上述药物对肝癌细胞增殖的抑制作用。 SPRi检测结果表明，苏拉明和顺铂能抑制肿瘤细胞HepG2增殖并呈现剂量、时间依赖关系。