海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备

摘要

The sulfated fuco-oligosaccharides were prepared via acid hydrolysis degradation of crude fucoidan extracted from Saccharina japonica. Through the optimization of degradation and precipitation conditions, the hydrolysis and sedimentation law of fucoidan were studied. Physicochemical analysis showed that the supernatant was heterozygous sulfated fuco-oligosaccharide(fucose>55%). Four fractions of fucose based low polyme-rization degree of oligosaccharides were purified by Bio-Gel P4 low-pressure permeation chromatogra-phy, and the fucose content of the fractions was up to 90% according to monosaccharide composition analysis and electrospray ionization mass spectrometry(ESI-MS) analysis. Thus, the fractions were series of low-poly-merized sulfated fuco-oligosaccharide / fucose, and the major fractions could be expressed with the formula of Fuc1~3 S1~3 , Fuc1~2 S1~2 , Fuc1~2 S, and FucGlcAS.%采用稀酸水解的方法降解粗褐藻多糖硫酸酯(FPS)制备了硫酸化岩藻寡糖.通过考察降解及沉降条件研究了FPS的水解及沉降规律.通过理化性质分析发现,沉降所得上层清液为杂合硫酸化岩藻寡糖[岩藻糖(Fuc)含量>55%].进一步采用Bio-Gel P4低压凝胶渗透色谱分离纯化,得到4种以岩藻糖为主的低聚合度硫酸化寡糖.单糖组成分析和电喷雾质谱(ESI-MS)分析结果表明,各组分主要为系列低聚合度硫酸化岩藻寡糖或硫酸化岩藻糖,包括Fuc1~3 S1~3,Fuc1~2 S1~2,Fuc1~2 S1及FucGlcAS.