采用背景荧光猝灭-免疫层析法(bFQICA),基于双抗体夹心法原理,将合适浓度的捕获抗体、 示踪抗体分别固定在试纸条和微孔内,层析时与样本形成抗体-抗原-抗体的夹心结构,建立了一种操作简便、 灵敏度高、抗干扰性强且能快速定量检测C-反应蛋白(CRP)的方法.结果表明,CRP在0.0～100.0 ng/mL浓度范围内与相对荧光强度值(F1/F2)呈现良好的相关性,最低检出限为0.0939 ng/mL,加标回收率为87.69％～111.0％,检测3批试剂的批间和批内相对标准偏差均小于15％,与降钙素原(PCT,20.0 ng/mL)及人血清淀粉样蛋白A(SAA,10.0μg/mL)均无交叉反应.采用该方法与免疫透射比浊法同时测定41例临床血清样本,检测结果相关性良好(r=0.9585,P<0.01),2种方法无显著性差异(P>0.05).%Background fluorescence immunochromatography assay( bFQICA) has been established, which has the advantages of simple operation, high sensitivity, strong anti-interference and rapid quantitative detection of C-reactive protein( CRP ) . This method of using double antibody sandwich method principle, the appropriate concentration of capture antibody and tracer antibodies are respectively fixed on the test strip and the micro-pore. During chromatography, antibodies were captured, while tracer antibodies and samples were inserted to form a sandwich structure of antibody antigen antibody. The method has a good correlation with fluorescence signal values(F1/F2) in the range of 0.0—100.0 ng/mL concentration in CRP, the minimum detection limit was 0.0939 ng/mL, and the recovery rate was 87.69％—111.0％, three batches of reagents and inter and intra assay relative standard deviation was less than 15％, and procalcitonin(PCT, 20.0 ng/mL), serum amyloid A like protein ( SAA, 10.0 μg/mL ) had not cross reaction with this method and the immune turbidimetric method for the simultaneous determination of 41 serum samples, the detection results had a good correlation(r=0.9585, P<0.01), there was no significant difference between the two methods(P>0.05). This method has high sensitivity, simple operation, and can be used for rapid and accurate quantitative detection of CRP .
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