周期蛋白B1和D1在重度反流性食管炎、Barrett食管和食管腺癌中的表达及相关性研究

摘要

目的 探讨周期蛋白(Cyclin)B1及D1在Barrett食管、Barrett食管合并不典型增生(DY)和食管腺癌中表达的临床意义.方法 应用免疫组织化学SP法测定68例患者食管组织标本Cyelin B1和Cyclin D1,其中重度反流性食管炎(RE)25例,Barrett食管(BE)35例,其中8例DY,8例食管腺癌(EA),另取10例正常食管黏膜组织作为对照.结果 Cyclin B1和Cyclin D1在BE、DY、EA组检测样本中均有高表达,而在正常对照组及RE组黏膜组织中仅有少量表达,差异有统计学意义(P<0.01),且Cyclin D1表达从肠化生-不典型增生.腺癌组织依次增高(分别为50.04、67.94、74.31),差异有统计学意义(P<0.01).结论 Cyelin B1和eyclin D1可以作为肿瘤进展标志物来评估Barrett食管患者进展为腺癌的危险性,并可能是食管腺癌发生过程中的早期事件.%Objective To explore the role of Cyclin B1 and Cyclin D1 on Barrett esophagus,Barrett's esophagus mixed with atypical dysplasia and esophageal adenocarcinoma.Methods Cyclin B1 and Cyclin D1 were examined with immunohistochemistry.76 esophageal tissues of patients werB collected,including severe reflux esophagitis(RE,25 cases),Barrett esophagus(BE,35 cases),Barrett esophagus mixed with atypical dysplasia(DY,8 cases),esophageal carcinoma(CA,8 cases).Ten cases with normal esophageal mucosa were examined as the control. Results Cyclin B1 and Cyclin D1 were high expression in the specimens of the BE,DY and CA groups and very low expression in the control and RE group.Statistieal difference Was showed(P<0.01).Expression of Cyclin D1 was increasing gradually from the tissues of intestinal metaplasia,atypical dysplasia to adenocarcinoma(50.04 vs 67.94 vs 74.31).There Was significant difference among these three groups(P<0.01).Conclusion Cyclin B1 and Cyclin D1 as markers of tumour development could evaluate the risk from Barrett esophagus to adenocarcinoma.Perhaps it is the earlv event in the development of esophageal carcinoma.