Protective Effect of Dimethyl-4,4'-Dimethoxy-5,6,5',6'-Dimethylene Dioxybiphenyl-2,2'-Dicarboxylate (DDB) against Carcinogen-Induced Rat Liver Nuclear DNA Damage

摘要

The protective effect fDDB against carcinogen-induced DNA damage was examined in the present investigation.Preincubation of rat liver nuclei with DDB(1mmol·L^-1)resulted in 60% inhibition of binding of ^3H-benzo(a)pyrene to nuclear DNA.unscheduled DNA synthesis(UDS)induced by aflatoxin B1(10^-7mol·L^-1) in freshly isolated rat hepatocytes was also inhibited by DDB(10^-6-10^-3mol·L^-1).Oral administation of DDB at 200mg·k^-1once daily for 3 d induced a significant increase of liver cytosol glutathione-S-transferase and microsomal UDPG-transferase activity in mice.These results indicate that DDB is able to directly or indirectly antagonize certain carcinogen-induced DNA damages.