首页> 外文期刊>生物医学与环境科学:英文版 >Evaluation of Cytogenetic Effects of Isoproturon on the Root Meristem Cells of Allium sativum
【24h】

Evaluation of Cytogenetic Effects of Isoproturon on the Root Meristem Cells of Allium sativum

机译:异丙隆对大蒜根分生细胞的细胞遗传学评价。

获取原文
获取原文并翻译 | 示例
       

摘要

Objective:To validate the use of Allium sativum sativum as a reliable test model for gentoxicity,isoproturon,a substituted phenylalkylures herbicide,was evaluated on the root meristem cells by this plant system.ethod :Test concentrations were selected by determining EC50 and root tips were exposed to various concentrations for 6 or 24hr.EC50 concentration was calculated to be 70.8ppm for the root growth.In addition on root growth retardation exposure to isoproturon induced morpholoogical changes like discoloruation and stiffness of roots.Results:Exposure to various experimental concentrations of isoproturon(35-280ppm),including EC50,significantly and dose-dependently inhibited the mitotic index and induced chromosome breaks/mitotic aberrations at 6 or 24 hr.Conclusion:The frequency of aberrations was found to be decreased in a dose dependant manner at 24 or 48hr post exposure.howerver in comparison of control cells the frequency of aberrations was considerably high which indicates genotoxicity potentials of isoproturon.Further,present study also suggests that Allium satiuvum is a sensitive,efficient,and reliable test system for measuring the genotoxicity potential of environmental chemicals.
机译:目的:为了验证利用大蒜作为遗传毒性的可靠测试模型,在该植物系统上对根分生组织细胞进行了异丙草隆,取代的苯基烷基脲类除草剂的评估。方法:通过确定EC50选择测试浓度并确定根尖暴露于各种浓度下6或24小时。计算得出根生长的EC50浓度为70.8ppm。此外,由于根生长迟缓,暴露于异丙肾上腺素引起的形态变化如根的变色和僵硬。结果:暴露于各种实验浓度的异丙肾上腺素(35-280ppm),包括EC50,在6或24小时时显着和剂量依赖性地抑制了有丝分裂指数并诱导了染色体断裂/有丝分裂畸变。结论:发现在24或24小时时,畸变的频率呈剂量依赖性降低。暴露后48小时。然而,与对照细胞相比,畸变的频率非常高,这表明遗传另外,目前的研究还表明,饱叶大蒜是一种灵敏,高效,可靠的测试系统,可用于测定环境化学物质的遗传毒性。

著录项

获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号