应用超声波辐射的方法,使壳聚糖和氯乙酸在NaOH-乙醇体系中反应,制备O-羧甲基壳聚糖(OCMC).测定了四因素三水平正交试验中的羧甲基取代度(DS)和氨基(N2-)保留量(％),优化了实验条件.选择较好的OCMC,以乙醇为溶剂,在超声波辐射下,通过和2,3-环氧丙基三甲基氯化铵(GTA)反应,合成具有抗菌性能的两性的季铵化O-羧甲基壳聚糖(QOCMC),并测定其季铵化取代度.红外光谱仪表征了其峰值的变化,扫描电镜表征其晶态结构的变化.通过对大肠杆菌和金黄色葡萄球菌最低抗菌浓度测试,得到QOCMC的最低抑菌浓度为OCMC的1/8,表明QOCMC的抗菌性能优于OCMC.%O-carboxymethyl chitosan (OCMC) was synthesized by ultrasonic irradiation with chitosan and chloroace-tic acid in NaOH-ethanol. The degree of substitution (DS) of carboxymethyl and amino retained amount (NH2%) were studied under the orthogonal experiments of four factors at three different levels and the reaction condition was optimized. Then quaternary ammonium group was introduced by the reaction of preferable OCMC with glycidyl tri-rnethylammonium (GTA) in ethanol. The DS of the quaternization group was studied. Antimicrobial activities of quatemized O-carboxymethyl chitosan (QOCMC) were evaluated against Eschenchta coli and Staphylococcus au-reus. The structures of the derivatives are characterized by FTIR spectrometer and SEM. The lowest antibacterial concentrations of QOCMC is 8 times lower than that of OCMC Experiment results validated that QOCMC is better than OCMC in the properties of antirr籧robial activity.
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