脂联素受体在人肾上腺皮髓质及其肿瘤中的不同表达

摘要

目的 观察脂联素受体1(AdipoR1)、脂联素受体2(AdipoR2)在正常肾上腺皮、髓质及其肿瘤中的mRNA表达.方法 提取6例正常人肾上腺皮质和髓质、10例皮质醇分泌瘤、14例醛固酮分泌瘤和20例嗜铬细胞瘤组织的总RNA,用RT-PCR方法检测AdipoR1、AdipoR2 mRNA表达;并分析AdipoR2与Adipo1的关系.31例患者曾行3 h葡萄糖耐量试验(OGTF),比较AdipoR1/R2与患者OGTT时血糖和胰岛素曲线下面积(GLU<,AUC>、INS<,AUC>)间的关系.结果 在肾上腺皮、髓质及其肿瘤中存在AdipoR1、AdipoR2的mRNA表达.AdipoR1 mRNA在正常肾上腺皮质内的表达明显低于皮质醇分泌瘤和醛固酮分泌瘤(P<0.01),AdipoR1 mRNA在嗜铬细胞瘤内的表达明显高于正常肾上腺髓质(P<0.01).AdipoR2 mRNA在正常肾上腺皮质内的表达明显低于皮质醇分泌瘤(P<0.05).皮质醇分泌瘤内AdipoR2 mRNA表达明显高于醛固酮分泌瘤(P<0.05).在所有组织中,AdipoR2与AdipoR1 mRNA表达高度相关r=0.335,P<0.01),AdipoR2 mRNA表达与GLU<,AUC>呈正相关(r=0.633,P<0.001).结论 肾上腺皮髓质及其肿瘤广泛表达脂联素受体并存在差异,可能与不同的脂联素水平相关.%Objective To compare the mRNA expression of adiponectin receptor 1 and adiponectin receptor 2 (Adipo R1/R2) in human adrenal tissues and tumors. Methods Total RNA was exracted from 6 normal human adrenal glands, 10 cortisol-secreting tumors, 20 pheochromacytomas and 14 aldosteronomas. We used RT-PCR to compare the mRNA expression of Adipo R1/R2 in human adrenal tissues and tumors and analyzed the relationship between Adipo RI/R2, the GLUAUc and INSAUC of 3 h OGTT. Results The mRNA expressions of AdipoR1/GAPDH and AdiPOR2/GAPDH were significantly lower in normal human adrenal cortex than that in aldosteronoma and cortisol-secreting tumors(P ＜0. 01 ). The mRNA expressions of AdipoR1/GAPDH in pheochromacytomas were higher than that in normal human adrenal medulla(P ＜0. 01 ). The mRNA expressions of AdipoR2/GAPDH in aldosteronoma were lower than cortisol-secreting tumors ( P ＜ 0. 05 ), Adipo R1 positively correlated with Adipo R2 ( r = 0. 335, P ＜ 0. 01 ),Adipo R2 positively correlated with GLUAUc(r =0. 633 ,P ＜0. 001 ). Conclusion Adiponectin was expressed in adrenal tissues, the difference may be explained by the plasma adiponectin.