目的 探讨不同浓度的角质细胞生长因子(KGF)对大鼠胰腺导管上皮细胞增殖的影响,寻求最佳浓度.方法 免疫细胞化学染色及RT-PCR方法鉴定SD大鼠胰腺导管上皮细胞；不同浓度的KGF刺激胰腺导管上皮细胞增殖,寻求最佳浓度.结果 大鼠胰腺导管上皮细胞表达Nestin和CK19,不表达Insulin及Glucagon;不同浓度KGF刺激胰腺导管上皮细胞2d后,细胞均有不同程度增殖,20 μg/L KGF作用2d时,细胞数为0.35±0.03,显著高于对照组的0.27±0.02(P ＜0.01).结论 KGF在本实验的剂量范围内可促进胰腺导管上皮细胞增殖,20 μg/L为促进增殖的最佳浓度.%Objective To investigate the effect of keratinocyte growth factor (KGF) at different concentrations on the proliferation of pancreatic ductal epithelial cells (PDECs). Methods The PDECs were identified by immuno-cytochemical stain and RT-PCR. PDECs counting was carried out at different KGF concentrations. Results Nestin and CK19 were positively expressed in PDECs by RT-PCR and immunocytochemical stain; PDECs were stimulated by different concentrations of KGF after 2 days, and PDECs number stimulated by 20μg/L KGF was 0. 35 ± 0. 03 , much higher than that of the control group which was 0. 27 ±0. 02. Conclusions KGF at different concentrations can significantly stimulate the proliferation of PDECs, the optimized concentration found in our tests is 20μg/L.
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