Evaluation of anti-apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide

摘要

Objective:To evaluate the anti-apoptotic and radical scavenging activities of dietary phenolics, namely ascorbic acid,a-tocopherol acetate,citric acid,salicylic acid,and estimate H₂O₂induced apoptosis in renal cell carcinoma cells.Methods:The intracellular antioxidant potency of antioxidants was investigated.H₂O-2-induced apoptosis in RCC-26 was assayed with the following parameters:cell viability（%apoptosis）,nucleosomal damage and DNA fragmentation, bcl-2 levels and flow cytometery analysis（ROS production evaluation）.Results:Ine anticancer properties of antioxidants such as ascorbic acid,a- tocopherol acetate,citric acid,salicylic acid with perdurable responses were investigated.It was observed that these antioxidants had protective effect（anti-apoptotic activity） against hydrogen peroxide（H₂O₂） in renal cell carcinoma（RCC-26） cell line.Conclusions:This study reveals and proves the anticancer properties.However,in cancer cell lines anti-apoptotic activity can indirectly reflect the cancer promoter activity through radicals scavenging,and significantly protect nucleus and bcl-2.