Inductively coupled plasma emission spectroscopic and flame photometric analysis of goat epididymal fluid

摘要

Aim: The elemental composition of the epididymal luminal fluid (ELF) in adult goat (Capra indica) was investigated. Methods: ELF was collected by micropuncture from twelve sites along the epididymal duct. The elemental contents was analyzed with inductively coupled plasma (ICP) emission spectroscopy, a microanalytical technique that can simultaneously measure many elements in minute volumes of sample. The Na and K concentrations were determined by flame photometry. Results: ICP spectroscopy showed the presence of copper, calcium, nickel, iron, magnesium, chromium, titanium and zinc in ELF, with fluctuating levels at different sites along the length of the epididymis.Cadmium, cobalt, lead and manganese were not found. The Na^+/K^+ ratio was seen to be higher at the initial segments of the epididymis and lower at the distal. Conclusion: It is proposed that the observed characteristic distribution of elements in ELF may have far reaching implications in sperm maturation and storage known to occur in the epididymis. (Asian J Androl 2000 Dec;2:288-292)