Effect of WNT5A on epithelial-mesenchymal transition and its correlation with tumor invasion and metastasis in nasopharyngeal carcinoma

摘要

Objective:To investigate the correlation between nasopharyngeal carcinoma cell WNT5A and epithelial-mesenchytnal transition(emt)/metastasis,and investigate its possible mechanisms.Methods:RT-PCR and gene transfection were used to detect the expression of nasopharyngeal carcinoma cell strains WNT5A and EMT related factor 5-8F.Transient transfcction of NPC cell line 5-8F was determined by liposome of plasmid with WNT5A gene.The differential expressions of WNTSA and EMT-related factors in cells before and after transfection were detected by RTPCR.Cell scratch assay and Transwell assay were used to detect the motility abilities of cells before and after 5-8F transfection.Results:The expressions of WNT5A and EMT related factors matrix metalloproleinasc-2 of the WNTSA transferred group in the nasopharyngeal carcinoma cell line 5-8F were higher than the blank control group and the empty vector transferred group,and the transfer ability of the WNTSA transferred group was higher than that in the blank control group and the empty vector transferred group,while the expressions of EMT related factors E-cadherin were lower than that in the blank control group and the empty vector transferred group,and the transfer ability of the WNT5A transferred group was higher than that in the blank control group and the empty vector transferred group.Conclusions:In nasopharyngeal carcinoma cells,WNT5A can regulate the epithelial-mesenchymal transition and affect the ability of tumor invasion and metastasis of nasopharyngeal carcinoma.