Antimicrobial and hemolytic activity of fish epidermal mucus Cynoglossus arel andArius caelatus

摘要

Objective:To study the antimicrobial, hemolytic activity and immunomodulatory activity of fish epidermal mucus and their chemical constituents fromCynoglossus arel (C. arel) and Arius caelatus (A. caelatus). Mucus plays an important role in the prevention of colonization by parasites, bacteria and fungi.Methods: Epidermal mucus was obtained from two marine fishes, lyophilized and the chemical composition of epidermal mucus was analysed byFT-IR analysis. Thein vitro antimicrobial activity against human pathogens (fungi, gram positive and gram-negative bacteria) and also the hemolytic activity and immunomodulatory activity were determined.Results:Totally ten human pathogens were tested against the fish mucus. Out of the ten pathogens, five pathogens have proved to be sensitive to the mucus. Maximum zone of inhibition was observed againstVibrio cholera (V. cholera) (9 mm and2 mm in diameter), followed byStaphylococcus aureus (S. aureus) with a inhibition zone of (6 mm and3 mm),Streptococcus areus (S. areus) (5 mm and4 mm),Vibrio parahemolyticus (V. parahemolyticus) (4mm and5 mm) respectively.Conclusions: The present investigation has revealed that positive progresses in the fish mucus extracts against human pathogens and hemolytic activity. But further efforts are required for the purification and isolation of the active antimicrobial compounds in order to establish their possible applications.