miR-218 expression in osteosarcoma tissues and its effect on cell growth in osteosarcoma cells

摘要

Objective:To investigate the expression of miR-218 and its clinical significance in osteosarcoma tissues and explore its effect on proliferation and apoptosis in osteosarcoma cells.Methods:miR-218 expression was detected in76 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues using quantitative reverse transcription polymerase chain reaction (qRT-PCR).MiR-218 was over-expressed by exogenous miR-218 plasmids inSaos-2 cells, and thenBrdU cell proliferation assay and flow cytometry were used to determine cell proliferation and apoptosis.Results:The expression of miR-218 in osteosarcoma tissues was significantly lower than those in normal tumor-adjacent tissues(t=8.735,P<0.001).MiR-218 expression in tumor tissues was significantly correlated with tumor size(χ2=5.380,P=0.020), clinical stage (χ2=6.692,P=0.010) and distant metastasis(χ2=4.180,P=0.041).MiR-218 was obviously over-expressed by exogenous miR-218 plasmids(t=19.42,P<0.001), and miR-218 overexpression significantly reduced cell proliferation(t=9.045,P<0.001) and induced apoptosis(t=12.38, P<0.001) inSaos-2 cells.Conclusions:The low-expression of miR-218 is correlated with the poor clinicopathological features in osteosarcoma.Moreover, miR-218 overexpression reduces cancer cell proliferation and induces apoptosis inSaos-2 cells, suggesting that miR-218 may play a key role in the progression of human osteosarcoma.