Effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells

摘要

Objective:To study the effects of regenerated tissue extracts after liver injury on the proliferation,differentiation,migration and invasion of SK-HEP 1 cells.Methods:Regenerated tissue extracts after liver injury were used to induce SK-HEP1 cells after enrichment,their effects on the proliferation,differentiation,migration and invasion of SK-HEP1 cells were observed through in vitro cell culture,MTT,flow cytometry and transwell assays.Results:In response to the action of regenerated tissue extracts after liver injury,SK-HEP1 cells were blocked in G0/G1 phase,their growth rate was distinctly reduced.The number of SK-HEP1-fj colonies decreased.The migration ability of SK-HEP1 cells showed a decreased trend on day 7 and day 11 after induction.SK-HEP1's invasion ability clearly decreased on days 7 and 11 after induction,especially on day 7.Conclusions:To a certain extent,regenerated tissue extracts after liver injury can inhibit the proliferation,differentiation,migration and invasion of hepatoma cells,showing an important potential of being a differentiating agent for the treatment of liver cancer.