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外文期刊>亚太热带生物医学杂志（英文版）
>Densitometric HPTLC analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams
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Densitometric HPTLC analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams
Objective: To develop and validate a simple, accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams. Methods:The analysis was performed on 10×20 cm aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F254 (E-Merck, Germany) with n-hexane: ethyl acetate 60: 40 (v/v) as mobile phase. Camag TLC Scanner III was used for the UV densitometric scanning at 569. Results:This system was found to give a compact spot of 8-gingerol at retention factor (Rf) value of (0.39±0.04) and linearity was found in the ranges 50-500 ng/spot (r2=0.9987). Limit of detection (12.76 ng/spot), limit of quantification (26.32 ng/spot), accuracy (less than 2 %) and recovery (ranging from 98.22-99.20) were found satisfactory. Conclusions:The HPTLC method developed for quantification of 8-gingerol was found to be simple, accurate, reproducible, sensitive and is applicable to the analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams.
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机译：目的：建立并验证一种简单，准确的HPTLC方法，用于分析生姜提取物和含姜的膳食补充剂，茶和市售面霜中的8-姜油，并测定8-姜油的含量。 方法：分析是在10×20 cm铝背板上进行的，涂有0.2 mm硅胶60 F254（E-Merck，德国），正己烷：乙酸乙酯60：40（v / v）作为流动相。 Camag TLC Scanner III用于569的紫外光密度扫描。范围50-500 ng / spot（r2 = 0.9987）。检出限（12.76 ng / spot），定量限（26.32 ng / spot），准确度（小于2％）和回收率（98.22-99.20）令人满意。 结论：HPTLC方法用于定量8-姜油被发现是一种简单，准确，可重现，灵敏的方法，适用于姜皮提取物和含姜的膳食补充剂，茶和商业食品中8-姜油的分析。面霜。
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