固定化混合菌处理含油废水优化实验

摘要

JA and JB,low temperature oil-degrading bacterias,were selected and domesticated from soil contaminated by oil. With the walnut shell as adsorption carrier,immobilized mixed bacteria was prepared for disposing oily wastewater, which was optimized by using the response surface method. The results showed that the degradation rate of immobilized mixed bacteria from this experiment on crude oil could reach a maximum of 69. 94% within 5 d, which is extremely close to the model predictive value of 68. 89%,while the number ratio of oil degradation bacteria-JA and JB was 1∶ 2,the mass concentration of crude oil was 4 300 mg/L,the pH value was 7. 9,the dosage of immobilized mixed bacteria was 27 g/L and the immobilizing time was 28. 5 h.%从受石油污染的土壤中筛选和驯化了两株耐低温石油降解菌JA和JB,以核桃壳为吸附载体,制备固定化混合菌,采用响应面法对固定化混合菌处理含油废水的条件进行优化。结果表明,在石油降解菌JA和JB活菌数量比为1∶2,原油质量浓度4300 mg/L,pH值7.9,固定化混合菌投加量27 g/L,固定化时间28.5 h条件下,固定化混合菌在5 d时间内对原油的降解率最高可达到69.94%,与模型预测值68.89%非常接近。